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目的 研究未致明显急慢性损伤剂量的超声能否增强阿霉素对卵巢癌细胞的细胞毒作用。方法 分 3组以克隆原细胞分析法分析各组细胞对阿霉素的反应性 ,仅以阿霉素处理组 (对照组 ,ADM)、超声辐照前给药组 (ADM +US)、超声辐照后给药组 (US +ADM)。并以荧光分析法测定 3组细胞细胞内药物聚积量。结果 ADM组、ADM +US组和US +ADM组IC50 分别为 0 .0 0 83 μg/ml、<0 .0 0 1μg/ml和0 .0 0 65 μg/ml,ADM +US组、US +ADM组克隆存活率均较ADM组低。与对照组比较 ,ADM +US组与US +ADM组克隆存活率降低 (P <0 .0 0 1) ,且ADM +US组克隆存活率明确低于US +ADM者。ADM +US组细胞内药物聚积量较ADM组高 (P <0 .0 1) ,US +ADM组细胞内药物聚积量未明显增加 (P >0 .0 5 )。结论 低频超声可增强阿霉素对卵巢癌细胞细胞毒作用 ,这种增效主要是通过提高细胞内药物聚积量实现
Objective To investigate whether the ultrasound that does not cause significant acute or chronic damage can enhance the cytotoxicity of doxorubicin on ovarian cancer cells. Methods Three groups of cells were analyzed by cloning cell analysis for doxorubicin responsiveness. Adriamycin-treated group (control group, ADM), pre-ultrasonic irradiation group (ADM + US), ultrasound After irradiation group (US + ADM). The amount of intracellular drug accumulation in three groups of cells was determined by fluorescence analysis. Results The IC50 of ADM group, ADM +US group and US +ADM group were 0. 0 0 83 μg/ml, <0. 0 0 1 μg/ml and 0. 0 0 65 μg/ml, respectively. ADM +US group, US + The ADM group had a lower survival rate than the ADM group. Compared with the control group, the survival rate of the clones in the ADM +US group and the US +ADM group decreased (P < 0.01), and the survival rate of the ADM +US group was clearly lower than that of the US +ADM group. The amount of intracellular drug accumulation in ADM +US group was higher than that in ADM group (P < 0.01), and there was no significant increase in intracellular drug accumulation in US +ADM group (P > 0.05). Conclusion Low-frequency ultrasound can enhance the cytotoxicity of adriamycin on ovarian cancer cells. This synergism is mainly achieved by increasing the amount of intracellular drug accumulation.