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目的观察狭基线纹香茶菜提取物对免疫性肝损伤小鼠TNF-α释放及肝组织超微结构的影响。方法 50只雄性NIH小鼠,随机分为5组,分别为正常对照组,模型组,狭基线纹香茶菜提取物高、低剂量组(剂量分别为18.2g/kg、4.55g/kg),与环磷酰胺(43mg/kg)对照组。除正常对照组外,其余小鼠于实验首日上午尾静脉注射刀豆蛋白A(ConA)20mg/Kg,并于首日、次日和第3d、第4d下午各给药1次,第5d给药后2h,模型组和各给药组小鼠再次尾静脉注射Con A 20mg/kg,4h后,每组取相同序号小鼠8只,取血检测血清TNF-α活性,每组所剩2只小鼠,8h后处死取肝组织,3%戊二醛固定,切片,染色后,透射电镜观察肝组织超微结构。结果显示模型组TNF-α活性较正常组明显升高,肝组织超微结构发生了显著改变,肝细胞严重肿胀、线粒体破坏、毛细胆管和肝窦腔结构明显变形、中性粒细胞滞留,细胞及细胞器的完整性遭到破坏。狭基线纹香茶菜提取物高、低剂量组TNF-α活性较模型组明显降低(P<0.01、0.05)、肝组织超微结构虽有一定程度改变,但细胞及细胞器的形态及完整性得到保护。结论狭基线纹香茶菜具有较好的保肝作用,其作用机理与抑制TNF-α的产生密切相关。
Objective To observe the effects of extracts of narrow-base line Hexaphylla on the release of TNF-α and ultrastructure of liver tissue in mice with immunological liver injury. Methods A total of 50 male NIH mice were randomly divided into 5 groups: normal control group, model group, and high-dose and low-dose group of narrow-rooted line incense tea extract (dose of 18.2g/kg and 4.55g/kg, respectively). , with cyclophosphamide (43mg/kg) control group. In addition to the normal control group, the remaining mice were injected with concanavalin A (ConA) 20 mg/Kg intravenously on the morning of the first day of the experiment and administered once on the first day, the next day, and on the 3rd and 4th afternoons, respectively. At 2 h after administration, Con A 20 mg/kg was injected into the tail of the mice in the model group and each administration group again. After 4 h, 8 mice of the same serial number were taken from each group and blood was taken to measure serum TNF-α activity. Two mice, 8 h later, were sacrificed to take liver tissue, fixed in 3% glutaraldehyde, sectioned and stained, and ultrastructure of liver tissue was observed by transmission electron microscope. The results showed that the TNF-α activity in the model group was significantly higher than that in the normal group, and the ultrastructure of the liver tissue was significantly changed. The hepatocytes were severely swollen, the mitochondria were destroyed, the bile ducts and hepatic sinus cavity structure were significantly deformed, and the neutrophils were retained. And the integrity of the organelles has been destroyed. The activity of TNF-α in the high-dose and low-dose groups of C. striata was significantly lower than that in the model group (P<0.01, 0.05). Although the ultrastructure of the liver tissue was changed to some extent, the morphology and integrity of the cells and organelles were reduced. Is protected. Conclusion The narrow-base line A. odorata has a good liver protection, and its mechanism of action is closely related to the inhibition of TNF-α production.