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The modified Cry1Ac was expressed in transgenic tobacco plants.To allow secretion of the Cry1Ac protein into the intercellular space,the signal peptide sequence of potato proteinase inhibitor Ⅱ(pinⅡ)was N-terminally fused to the CrylAc encoding region.Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA.The results showed that pinⅡ signal peptide sequence enhanced the expression of Cry 1 Ac protein and led to the secretion of the Cry 1Ac protein in transgenic tobacco plants.GFP gene was also fused to the signal peptide sequence and transformed to tobacco.The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.