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目的研究肝硬化患者血清中Micro RNA-122含量与肝硬化分级的相关关系,为Micro RNA-122用于肝硬化分级提供实验依据。方法实时荧光定量PCR法测定78例肝硬化患者血清中Micro RNA-122的含量。肝硬化患者根据Child-Pugh进行分级。比较各组间Micro RNA-122和肝硬化分级的关系。结果肝硬化失代偿期与代偿期的血清mi R-122的Ct值差异具有统计学意义(37±3 vs.26±4,P=0.002);腹水与无腹水肝硬化患者血清mi R-122的Ct值差异具有统计学意义(36±3 vs.27±2,P=0.002);上消化道出血与无上消化道出血患者血清mi R-122的Ct值差异具有统计学意义(38±3 vs.23±3,P=0.002);有自发性细菌性腹膜炎和没有的患者血清mi R-122的Ct值差异具有统计学意义(36±4 vs.25±2,P=0.002),没有这些并发症的肝硬化患者mi R-122水平显著降低。Child-Pugh A级与Child-Pugh B级肝硬化患者血清mi R-122的Ct值没有统计学差异(22±3 vs.27±5,P=0.200),Child-Pugh A级患者血清mi R-122的Ct值显著低于Child-Pugh C级患者(22±3 vs.41±3,P=0.001),同时Child-Pugh B级患者血清mi R-122的Ct值显著低于Child-Pugh C级患者(27±5 vs.41±3,P=0.001)。结论血清中Micro RNA-122含量可作为肝硬化患者分级的生物标记物。
Objective To study the relationship between Micro RNA-122 level and cirrhosis grade in patients with cirrhosis and to provide experimental evidence for the classification of Micro RNA-122 for cirrhosis. Methods The contents of Micro RNA-122 in sera of 78 cirrhotic patients were determined by real-time fluorescence quantitative PCR. Patients with cirrhosis were graded according to Child-Pugh. The relationship between MicroRNA-122 and cirrhosis grade was compared between groups. Results There was a significant difference in Ct values between patients with decompensated cirrhosis and compensated serum mi R-122 (37 ± 3 vs.26 ± 4, P = 0.002); ascites with serum ascites without cirrhosis -122 had statistical significance (36 ± 3 vs.27 ± 2, P = 0.002). There was significant difference in Ct values of serum mi R-122 in patients with upper gastrointestinal bleeding and upper gastrointestinal bleeding ( 38 ± 3 vs.23 ± 3, P = 0.002). There was significant difference in Ct values of serum mi R-122 between patients with and without spontaneous bacterial peritonitis (36 ± 4 vs.25 ± 2, P = 0.002 ), Mi R-122 levels were significantly reduced in cirrhotic patients without these complications. The Ct values of serum mi R-122 in Child-Pugh class A and Child-Pugh class B cirrhosis patients were not significantly different (22 ± 3 vs.27 ± 5, P = 0.200) -122 was significantly lower than that of Child-Pugh C (22 ± 3 vs.41 ± 3, P = 0.001), while the Ct of serum mi R-122 in Child-Pugh class B was significantly lower than that of Child-Pugh Grade C patients (27 ± 5 vs. 41 ± 3, P = 0.001). Conclusion Micro RNA-122 levels in serum can be used as biomarkers for the classification of patients with cirrhosis.