[目的]探索利用顺铂联合放疗对胶质瘤治疗复发率的影响。[方法]将C6细胞分为空白对照组、单纯放疗组、单纯化疗组和放疗加化疗组,激光共聚焦观察细胞形态,用流式细胞仪测定CFSE的荧光信号。[结果]放疗加化疗组细胞的增殖能力较单纯放疗及单纯化疗组明显下降。[结论]放疗加化疗联合不仅减少了化疗药物和射线的用量,也大大降低了治疗后的肿瘤细胞增殖能力,为指导临床胶质瘤的后续治疗提供依据。 [Objective] To explore the use of cisplatin combined with radiotherapy on the treatment of glioma recurrence rate. [Methods] The C6 cells were divided into blank control group, radiotherapy alone group, chemotherapy alone group and radiotherapy plus chemotherapy group. The morphological changes of CFSE cells were observed by laser scanning confocal microscope. Fluorescence signals of CFSE were measured by flow cytometry. [Results] The proliferation of radiotherapy plus chemotherapy group was significantly lower than that of radiotherapy and chemotherapy alone group. [Conclusion] Combination of radiotherapy and chemotherapy not only reduces the dosage of chemotherapy drugs and radiation, but also greatly reduces the proliferation ability of tumor cells after treatment, so as to provide basis for guiding the follow-up treatment of clinical glioma.