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目的建立高效液相色谱法同时测定石榴健胃丸中桂皮醛、胡椒碱含量的方法。方法色谱柱采用CAPCELL PAKC18(4.6 mm×250 mm,5μm)柱;流动相为乙腈-水(40∶60);流速:1.0 mL.min-1;检测波长:290 nm;柱温:30℃。结果桂皮醛在1.149 5～13.793 5μg之间呈良好的线性关系,平均回收率为97.27%,RSD为1.39%(n=6);胡椒碱在0.726 4～2.724μg之间呈良好的线性关系,平均回收率为96.51%,RSD为1.39%。结论本法简便、准确,专属性强,可作为控制石榴健胃丸质量的方法。
Objective To establish a method for simultaneous determination of cinnamaldehyde and piperine in Pomegranate Jianwei Pills by high performance liquid chromatography. Methods The column was equipped with a CAPCELL PAKC18 column (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile-water (40:60). The flow rate was 1.0 mL.min-1. The detection wavelength was 290 nm. Results Cinnamaldehyde showed a good linear relationship between 1.149 5 and 13.793 5 μg with an average recovery of 97.27% and a RSD of 1.39% (n = 6). Piperine showed a good linear relationship between 0.726 4 and 2.724 μg, The average recovery was 96.51% with a RSD of 1.39%. Conclusion This method is simple, accurate and specific and can be used as a method to control the quality of Pomegranate Jianwei pills.