目的　寻找可同时分离酸性和碱性蛋白的合适缓冲液。方法　以马心肌红蛋白、β -乳球蛋白A、碳酸酐酶II和核糖核酸酶A为标准混合样品 ,详细研究了四种缓冲液 ,磷酸盐、硼酸盐、Tricine和Tris对毛细管区带电泳分离蛋白质的影响。结果　磷酸盐缓冲液与毛细管壁和蛋白均可发生作用 ,能改变碱性蛋白质的迁移规律 ;而硼砂缓冲液主要是通过与毛细管壁作用抑制蛋白的吸附 ,不改变蛋白质的迁移规律 ;Tris和Tricine不能有效地抑制蛋白质的吸附。结论　磷酸盐是同时分离酸性和碱性蛋白质的较好缓冲液。 Aim To find suitable buffers that can separate acidic and basic proteins simultaneously. Methods The myoglobin, β - lactoglobulin A, carbonic anhydrase II and ribonuclease A were used as the standard mixed samples. The effects of four buffers, phosphate, borate, Tricine and Tris on the capillary zone Influence of protein separation by electrophoresis. Results Both phosphate buffer and capillary wall and protein could play an important role in altering the migration of basic proteins. Borax buffer mainly inhibited the protein adsorption by capillary action and did not change the protein migration. Tris and Tricine Can not effectively inhibit protein adsorption. Conclusion Phosphates are a good buffer for the simultaneous separation of acidic and basic proteins.