甲型肝炎病毒（HAV）不仅可适用于人和非人灵长目动物细胞,还可适应于一些非灵长目动物细胞,病毒复制不同步和+RNA极易装壳耗损是导致病毒低产量的主要原因。野型HAV的细胞适应和减毒实质上是病毒核酸5′非编码区和P2-P3功能区的突变过程,检查核酸序列可作为疫苗质控的遗传标记。本文对提高甲型肝炎疫苗产量,开拓质控指标具有参考意义。 Hepatitis A virus (HAV) is not only suitable for human and non-human primate cells, but also adapted to some non-primate cells. Asynchronous replication of virus and extreme wear of + RNA are the main reasons leading to low virus yield. The cellular adaptation and attenuation of wild-type HAV is essentially the process of mutation of the 5 ’non-coding region and the P2-P3 functional region of the viral nucleic acid and the checking nucleic acid sequence serves as a genetic marker for vaccine quality control. This article has the reference meaning to raise the Hepatitis A vaccine output, develops the quality control index.