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目的：应用抗ｂＦＧＦ单克隆抗体研究小鼠肝脏癌前病变中ｂＦＧＦ的表达。方法：将津白２纯系小白鼠６０只随机分成三组：ＡＦＢ１组，ＡＦＢ１＋香烟熏组和对照组。ＡＦＢ１溶于ＤＭＳＯ，５ｕｇ／日，只量拌于饲料投食两实验组，熏烟组另以烟丝３０ｇ／日熏烟，经半年实验，取肝脏切片行免疫组化染色。使用医学图像处理系统检测ｂＦＧＦ表达强度。结果：三组小鼠肝ｂＦＧＦ表达强度呈ＡＦＢ１组＞熏烟组＞对照组的趋势，各组间差异均具有显著性意义（Ｐ＜００５），且ｂＦＧＦ表达与ＤＮＡ含量呈正相关。我们推测：ＡＦＢ１诱导的小鼠肝脏癌前病变与ｂＦＧＦ表达上调所至细胞增殖有关。结论：本实验从另一侧面证实香烟具有阻止ＡＦＢ１诱导小白鼠肝癌变的作用
OBJECTIVE: To study the expression of bFGF in mouse premalignant lesions using anti-bFGF monoclonal antibody. Methods: 60 Jinchun 2 pure white mice were randomly divided into three groups: AFB1 group, AFB1+ cigarette smoke group and control group. AFB1 was dissolved in DMSO for 5ug/day and was only mixed in the experimental group and the experimental group. The smoked group was treated with 30g/day smoked tobacco. After half a year of experiment, the liver sections were taken for immunohistochemical staining. The bFGF expression intensity was measured using a medical image processing system. RESULTS: The expression of bFGF in livers of the three groups showed the trend of AFB1> fumigation group> control group. The differences among the groups were significant (P < 0.05), and the expression of bFGF was positively correlated with DNA content. We speculate that AFB1-induced liver precancerous lesions are associated with upregulation of bFGF expression to cell proliferation. Conclusion: This experiment confirms from another side that cigarettes have the effect of preventing AFB1-induced hepatocarcinogenesis in mice.