世界卫生组织报导:各国5岁以下儿童腹泻病例中,由轮状病毒引起的约占50％,鉴于本病在幼儿中广泛传播,为了解托幼机构外环境轮状病毒污染情况,特进行本试验。方法选择海珠区两个卫生院(宝岗、海幢)管段的16个托幼机构,于1985年4月份进行调查:儿童手(每20个小孩为一样本)、桌面、椅面、地面,玩具、门把手、床、茶杯等(每采样约涂搽500cm~2),用灭菌棉拭子采样后洗入1.5ml 0.02M磷酸盐缓冲液中,3000次/分离心20分钟,取上清液,用兰州生物制品 According to the World Health Organization (WHO), about 50% of cases of diarrhea among children under the age of 5 in the world are caused by rotavirus. In view of the widespread transmission of this disease in young children, in order to understand the rotavirus contamination outside the kindergarten organization, test. Methods Sixteen nurseries were selected from two hospitals (Baogang, Haikua) in Haizhu District in April 1985. Children’s hands (one for every 20 children), tabletop, chair surface, Toys, door handles, beds, cups, etc. (about 500cm ~ 2 for each sample), sample with sterilized cotton swab and wash in 1.5ml 0.02M phosphate buffer, 3000 times / centrifugation for 20 minutes, Serum, with Lanzhou biological products