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本实验发现Cu(Ⅰ )与H2 O2 反应生成羟自由基的产率比Fenton试剂的高 1 0倍以上。采用苯基荧光酮(Phenylfluorone,简称苯芴酮 ) -Cu(Ⅰ )—H2 O2 荧光法测定羟自由基的新体系 ,实验选择激发波长和发射波长分别为 50 0nm和 4 2 0nm ,测定苯基荧光酮在反应前后的荧光变化 ,即可间接测定羟自由基的产生量。此法不需要昂贵的仪器设备 ,方法灵敏 ,且操作简便易行。清除率实验及顺磁共振波谱法检测证明了该方法的准确可靠 ,对于医用筛选抗羟自由基及抗羟自由基机理研究等方面具有应用价值。
The experiment found that Cu (Ⅰ) reacted with H2O2 to generate hydroxyl radical in a yield 10 times higher than that of Fenton reagent. A new system for the determination of hydroxyl radical by Phenylfluorone-Cu (Ⅰ) -H2 O2 fluorescence method was used. The excitation and emission wavelengths were chosen as 50 0 nm and 420 nm respectively. Fluorescence ketone fluorescence changes before and after the reaction, you can indirectly determine the amount of hydroxyl radicals generated. This method does not require expensive equipment, sensitive method, and easy to operate. The clearance rate experiment and paramagnetic resonance spectroscopy test proved the method is accurate and reliable, which has the application value in the medical screening of the anti-hydroxyl radical and the study of the mechanism of anti-hydroxyl radical.