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背景:连续高浓度吸氧可以提高大鼠抗氧化酶,同时对肺产生损伤作用。目的:观察间断、高浓度吸氧对大鼠抗氧化酶活性及肺组织的影响。设计:完全随机设计,对照实验。单位:解放军总医院老年心内科和生化科。材料:实验于2002-01/2002-05在海军总医院高压氧科和解放军总医院生化科内完成。选用健康Wister大鼠10只,体质量200～250g,雌雄不拘。随机将大鼠分为2组,每组5只。方法:对照组大鼠在室内环境喂养7d。高氧预适应组动物放入高压氧仓内,每日吸80%～85%的氧气(101.325kPa)6h,连续7d。实验结束后取大鼠肺称肺湿重。并取肺组织0.4g,在冰浴上制成匀浆,取上清液采用化学发光法分别测定超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性。将肺组织采用30g/L戊二醛固定,制成切片后电镜下观察肺脏超微结构。主要观察指标:①两组大鼠肺湿重及肺组织超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性比较。②肺组织超微结构改变。结果:大鼠10只均进入结果分析。①肺组织超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性:高氧预适应组均明显高于对照组[(185.10±1.92)%/g,(90.50±4.17)nkat/g,(4.38±0.18K/g;(95.15±1.43)%/g,(20.17±2.17)nkat/g,(1.10±0.19)K/g,t=-84.016,-33.488,-28.023,P<0.01]。②大鼠肺湿重:高氧预适应组与对照组相近[(1.32±0.07),(1.30±0.03)g,P>0.05],说明高氧预适应组肺内液体没有增加。③肺组织超微结构:高氧预适应组肺组织基膜完整、厚度均匀一致,血管内皮细胞完整,Ι型上皮细胞无破坏。结论:间断、高浓度吸氧能提高大鼠肺内抗氧化酶活性,对大鼠肺无明显的损伤作用。
Background: Continuous high concentration of oxygen can increase rat anti-oxidant enzymes, while damaging the lungs. Objective: To observe the effects of intermittent and high concentration oxygen on antioxidant enzyme activity and lung tissue in rats. Design: completely random design, control experiment. Unit: Department of Geriatrics, General Hospital of People’s Liberation Army and Department of Biochemistry. MATERIALS: The experiment was performed in the Department of Biochemistry of the Navy General Hospital and PLA General Hospital from January 2002 to May 2002. Selection of healthy Wister rats 10, weight 200 ~ 250g, male or female. Rats were randomly divided into two groups of five. Methods: The control group rats were fed in the indoor environment for 7 days. Hypoxic preconditioning group of animals into the hyperbaric oxygen chamber, inhaling 80% to 85% oxygen (101.325kPa) 6h, 7d consecutive days. After the experiment, the lungs of rats were weighed and weighed. 0.4g of lung tissue was taken and homogenized in an ice bath. The supernatants were assayed for superoxide dismutase, glutathione peroxidase and catalase activity by chemiluminescence. The lung tissue was fixed with 30g / L glutaraldehyde, and the lung ultrastructure was observed under electron microscope. MAIN OUTCOME MEASURES: ① lung wet weight and lung tissue superoxide dismutase, glutathione peroxidase and catalase activity in two groups were compared. ② lung ultrastructure changes. Results: Ten rats were involved in the result analysis. (1) The activities of superoxide dismutase, glutathione peroxidase and catalase in lung tissue were significantly higher in hyperoxia preconditioning group than those in control group [(185.10 ± 1.92)% / g, (90.50 ± 4.17) nkat / g, (4.38 ± 0.18 K / g; 95.15 ± 1.43% / g, 20.17 ± 2.17 nkat / g, 1.10 ± 0.19 K / g, t = -84.016, -33.488, -28.023, P <0.01] .②The lung wet weight of rats: The Hypoxic preconditioning group was similar to the control group [(1.32 ± 0.07), (1.30 ± 0.03) g, P> 0.05] Increased.③The ultrastructure of lung tissue: The preconditioning group had a complete basement membrane and uniform thickness, the vascular endothelial cells were intact, and the type I epithelial cells were not damaged.Conclusion: Intermittent and high concentration of oxygen can improve the intrapulmonary Antioxidant enzyme activity, no significant damage to rat lung.