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收集L·F·B染色阳性肝癌,肝硬化、肝炎肝活检标片18例。经醛复红、地依红、HBsAg,PAP证实4例肝炎后肝硬化,2例肝癌中有乙型肝炎病毒包涵体,余10例门脉性肝硬化和2例慢性活动性肝炎胞浆内有L·F·B单独阳性包涵体,认为是NANB病毒包涵体。并认为L·F·B染色时NANB肝炎病毒包涵体具有特异性。此外,经电镜观察证明,在一例肝硬化病例的肝细胞装中见有NANB肝炎病毒样颗粒,进一步证实L·F、B染色对NANB型肝炎病毒包涵体的特异性。
Eighteen cases of L · F · B stained positive liver cancer, liver cirrhosis and hepatitis liver biopsy were collected. Four cases of posthepatitic cirrhosis were confirmed by aldehyde complex red, red blood, HBsAg and PAP, two cases of hepatocellular carcinoma with hepatitis B virus inclusion bodies, the other 10 cases of portal cirrhosis and two cases of chronic active hepatitis There is a L · F · B single positive inclusion body, which is considered NANB virus inclusion body. It is considered that the NANB hepatitis virus inclusion body is specific for L · F · B staining. In addition, electron microscopic observation confirmed that NANB hepatitis virus-like particles were found in the liver cell of a case of cirrhosis, further confirming the specificity of L · F and B staining for NANB hepatitis virus inclusion bodies.