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本试验选用抗病性较强的球茎大麦(2n=28)为父本,以普通小麦(2n=42)为母本,进行属间杂交,授粉24小时后,在母本柱头上滴赤霉酸,促使幼胚生长,以中国春单体5B 为母本杂交,获得了少量结实的杂交种子;以中国春为母本的,则结合离体培养杂种幼胚,以获得属间杂种。杂种自花不育,而以中国春单体5B 与之回交,获得三株回交一代杂种(B_1F_1)。杂种 F_1与 B_1F_1代的植株性状有明显的杂种优势; 二者穗部形态结构基本相似,均为穗形细长、有芒似父本,而小穗之多花性则似母本;F_1和 B_1F_1与其父本球茎大麦一样对赤霉病和白粉病等严重的小麦病害具有抗性。观察其染色体数,F_1在24～30之间,而B_1F_1染色体数在45～49之间(其中大多数终变期细胞有20～21个配对的二价体,3～7个单价体)。B_1F_1代自交与回交均有部分结实。预计经过继续自交和回交,可能在后代中获得抗病的小麦异附加系。
In this experiment, the bulbs of barley (2n = 28) with strong disease resistance were used as the male parent, and the common wheat (2n = 42) was used as the female parent for interspecific hybridization. After 24 hours of pollination, Acid, promote the growth of immature embryos, the Chinese spring single plant 5B as the female parent to obtain a small amount of hybrid seed; with Chinese spring as the female, then combined with in vitro hybrid embryos to obtain intergeneric hybrids. Hybrid self-sterile, but to China Spring monomer 5B with the backcross, access to three backcross generation hybrids (B_1F_1). The hybrid plants of F_1 and B_1F_1 hybrids had obvious heterosis. The morphological structures of the two panicles were similar and spikelets were slender, B_1F_1, like its male parent bulbous barley, is resistant to severe wheat diseases such as scab and powdery mildew. The number of chromosomes was observed, F_1 was between 24 and 30, while the number of B_1F_1 chromosomes was between 45 and 49 (most of the final cells had 20-21 paired bivalents and 3 or 7 monovalents). B_1F_1 self-made and backcrossed both have some solid. It is expected that resistant wheat lines will be acquired in offspring through continuous selfing and backcrossing.