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利用硫氰酸胍裂解病毒和二氧化硅颗粒能够特异性吸附核酸的特性，将二氧化硅悬液、裂解液及肝炎病毒感染血清共同保温，病毒裂解释放出的核酸结合在二氧化硅颗粒上，经过双蒸水洗脱，所得核酸直接作ＰＣＲ扩增。以该法提取感染血清样本中的ＨＢＶＤＮＡ或ＨＣＶＲＮＡ作为模板，再进行ＰＣＲ扩增检测，产物在琼脂糖凝胶电泳上所见条带清晰。用本法检测ＨＢｓＡｇ（＋）／ＨＢｅＡｇ（＋）Ａｕｔｉ－ＨＢｃ（＋）血清３２例，阳性率为９６％；检测抗－ＨＣＶ阳性血清１１０例，阳性率为８２．７％。该方法成本低，实验周期短，结果稳定。
The use of guanidine thiocyanate lytic virus and silica particles can be specific adsorption of nucleic acid characteristics, the suspension of silica, lysate and hepatitis virus infection sera co-incubation, viral lysis of the release of nucleic acid bound to the silica particles , After double distilled water, the resulting nucleic acid directly for PCR amplification. The HBVDNA or HCV RNA in the infected serum samples was extracted by this method as a template and then amplified by PCR. The bands of the product were clear on agarose gel electrophoresis. 32 cases of HBsAg (+) / HBeAg (+) Auti-HBc (+) serum were detected by this method, the positive rate was 96%. The positive rate of anti-HCV positive serum in 110 cases was 82.7%. The method has the advantages of low cost, short experiment period and stable result.