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目的通过检测三氧化二砷(As2O3)对肝癌SMMC-7721细胞周期素依赖性激酶抑制剂(CDKI)p27kip1及S期激酶相关蛋白2(Skp2)的影响,探讨其抗癌作用机制。方法体外培养人肝癌细胞株SMMC-7721,用2μmol/LAs2O3处理72h,流式细胞仪检测细胞周期变化及细胞凋亡率,应用Westernblot方法检测p27kip1、Skp2及CDK2基因编码蛋白的表达。结果与对照组比较,As2O3使SMMC-7721细胞周期阻滞在G2/M期。经As2O3作用的人肝癌细胞p27kip1基因编码蛋白表达增加,而p27kip1相关分子Skp2及CDK2的表达减少。结论As2O3可干扰细胞周期的进程,抑制SMMC-7721细胞的增殖。其作用机制与上调p27kip1蛋白及下调Skp2、CDK2有关。
Objective To investigate the effect of arsenic trioxide (As 2 O 3) on the expression of p27kip1 and S-kinase 2 (Skp2) in hepatocellular carcinoma cell line SMMC-7721 and its anti-cancer mechanism. Methods Human hepatocellular carcinoma cell line SMMC-7721 was cultured in vitro and treated with 2μmol / L As2O3 for 72h. The cell cycle and apoptosis rate were detected by flow cytometry. The protein expressions of p27kip1, Skp2 and CDK2 were detected by Western blot. Results Compared with the control group, As2O3 reduced the cell cycle of SMMC-7721 in G2 / M phase. The expression of p27kip1 gene in As2O3-treated human hepatocellular carcinoma cells increased while the expression of p27kip1-related molecules Skp2 and CDK2 decreased. Conclusion As2O3 can interfere with the cell cycle progression and inhibit the proliferation of SMMC-7721 cells. Its mechanism of action and up-regulation of p27kip1 protein and down-regulation of Skp2, CDK2.