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胃癌是我国常见癌瘤之一。研究胃癌细胞的超微结构,对探索胃癌细胞的来源及评价治疗效果等有一定价值。有关胃癌细胞的超微结构特征及放疗后的超微结构改变,国内外报道不多。现将我们的观察结果报告如下。 材料和方法 一、材料 胃癌手术切除标本或胃镜活检标本共20例,其中10例手术前用加速器照射,总量为3,500～4,000rad,照射后10～14天取材。 二、标本制作 所取标本立即用3％戊二醛PBS溶液固定(4℃)3小时,以1/15M蔗糖磷酸缓冲液冲
Gastric cancer is one of the common cancers in China. The study of the ultrastructure of gastric cancer cells has certain value for exploring the source of gastric cancer cells and evaluating the therapeutic effect. The ultrastructural characteristics of gastric cancer cells and the ultrastructural changes after radiotherapy have not been reported at home and abroad. We now report our observations as follows. Materials and Methods I. Materials Gastric cancer surgical specimens or gastroscope biopsy specimens of a total of 20 cases, including 10 cases with an accelerator before surgery, the total amount of 3,500 ~ 4,000rad, 10 to 14 days after irradiation materials. Specimen Preparation The specimens were immediately fixed with 3% glutaraldehyde in PBS (4°C) for 3 hours and flushed with 1/15M sucrose phosphate buffer.