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小鼠腹水癌细胞经PN-5Fu加光辐照处理后,癌细胞脂质过氧化产物丙二醛(MDA)、形成增多,随光照时间延长,其形成量显著增加(P>0.05-0.01),荷瘤小鼠局部肿瘤经PN-5Fu光动力治疗后,肿瘤组织中MDA含量也明显高于对照纽。(P<0.05),提示PN-5Fu光化学反应可以引起癌细胞膜磷脂中多不饱和脂肪酸的脂质过氧化。用苯甲酸捕获·OH的荧光法测定表明,PN-5Fu光动力反应过程中有羟自由基生成。荧光偏振方法测定细胞膜脂流动性的结果显示PN-5Fu的光动学作用可使腹水癌细胞膜的微粘度明显增高,反映膜脂的流动性明显下降。由透射电镜观察到PN-5Fu光动力治疗作用可使癌细胞超微结构发生显著变化,光照1小时后癌细胞胞浆疏松,线粒体肿胀、空泡变性;光照6小时后可见癌细胞膜破坏,核浓缩、碎解、甚至捎失。实验还表明,PN-5Fu光动学作用所致皮肤光敏反应可完全被维生素E所阻断,苯海拉明及消炎痛均能对抗PN-5Fu的皮肤光敏反应。
After treated with PN-5Fu plus light irradiation, the malondialdehyde (MDA) formation and formation of lipid peroxidation in cancer cells of mice with ascites cancer cells increased, and the amount of malondialdehyde (MDA) increased significantly with the prolonged illumination time (P>0.05-0.01). After photodynamic treatment with PN-5Fu in tumor-bearing mice, the MDA content in the tumor tissue was also significantly higher than that of the control New Zealand. (P<0.05), suggesting that photochemical reaction of PN-5Fu can cause lipid peroxidation of polyunsaturated fatty acids in the membrane phospholipids of cancer cells. The fluorometric determination of OH with benzoic acid indicates that hydroxyl radicals are generated during the photodynamic reaction of PN-5Fu. The results of fluorescence polarization measurement of cell membrane lipid fluidity showed that the photodynamic effect of PN-5Fu could significantly increase the microviscosity of ascites cancer cell membrane, indicating that the fluidity of membrane lipid was significantly decreased. Transmission electron microscopy showed that the photodynamic therapy of PN-5Fu could significantly change the ultrastructure of cancer cells. After 1 hour of light irradiation, the cytoplasm was loose, mitochondrial was swollen, and vacuolar degeneration was observed; after 6 hours, the destruction of cancer cell membrane was observed. Concentrate, break, or even lose. The experiment also showed that the photosensitivity of skin caused by PN-5Fu’s photodynamic action can be completely blocked by vitamin E, and both diphenhydramine and indomethacin can resist the skin photosensitivity reaction of PN-5Fu.