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Objective:To study the effects and mechanism of homocysteine(Hcy)on Parkinson’s disease(PD)induced by 6-hydroxydopamine(6-OHDA)in vivo.Methods:Forty rats were divided into 4 groups.6-OHDA or the solvent of 6-OHDA was focally administrated to induce PD,2 h later Hcy or 0.9% sodium chloride was administrated in the ipsolateral substantial nigra(SN).We used behavioral testing,Immolunohistochemical techniques,biochemistry techniques to detect the injury of SN.Results:The rotary turns of PD rats induced by 6-OHDA showed significant increase after treatment with Hcy compared with the controls(P < 0.05).Also the numbers of tyrosine hydroxylase(TH)-stained neurons were decreased,and dendrites were fragmented and truncated.Free radicals were increased and antioxidant enzymes decreased.Conclusion:Focal infusion of Hcy into the SN increased the vulnerability of the dopaminergic neurons to 6-OHDA-induced degeneration,it seems that the endangering effect of Hcy is due to exacerbating oxidative stress.
Objective: To study the effects and mechanism of homocysteine (Hcy) on Parkinson’s disease (PD) induced by 6-hydroxydopamine (6-OHDA) in vivo. Methods: Forty rats were divided into 4 groups. -OHDA was focally administered to induce PD, 2 h later Hcy or 0.9% sodium chloride was administrated in the ipsolateral substantial nigra (SN). We used behavioral testing, Immolunohistochemical techniques, biochemistry techniques to detect the injury of SN. Results: The rotary turns of PD rats induced by 6-OHDA showed significant increase after treatment with Hcy compared with the controls (P <0.05). Als the numbers of tyrosine hydroxylase (TH) -stained neurons were decreased, and dendrites were fragmented and truncated. were increased and antioxidant enzymes decreased. Confocal: Focal infusion of Hcy into the SN increased the vulnerability of the dopaminergic neurons to 6-OHDA-induced degeneration, it seems that the endangering effect of Hcy is due to exacerbating oxidative stre ss.