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目的 探讨梭曼染毒自由基损伤及维生素A(VA)和维生素E(VE)对该损伤的保护作用。方法 实验设对照组 ,梭曼染毒模型组及VA 和VE 实验组。在梭曼染毒前 ,对照组和梭曼染毒模型组ig处理过的菜子油 (1mL·kg- 1·d- 1) ,VA 及VE 实验组分别按VA2mg·kg- 1和VE2 .5mg·kg- 1的剂量ig ,共 9d。d 10除对照组外 ,其余大鼠均用梭曼腹部sc(6 3μg·kg- 1) ,中毒 2h后宰杀取血和肝脏组织 ,观察大鼠血清和肝组织中相关指标的变化。结果 结果显示 ,染毒模型组AChE严重下降 ,血清和肝组织中MDA含量明显升高 ,血清和肝组织中SOD活性下降 ,全血和肝组织中GSH Px活性呈显著下降趋势。VA 和VE 实验组血清MDA含量下降 ,在血清和肝组织中SOD及全血和肝组织中GSH Px活性均上升。结论 梭曼染毒引起AChE活性严重下降 ,同时伴有严重的自由基损伤 ,染毒前大鼠用VA 和VEig处理 ,能够保护AChE活性 ,同时作为抗氧化剂的VA和VE 能保护梭曼染毒所致的自由基损伤
Objective To investigate the protective effects of soman on free radical injury and vitamin A (VA) and vitamin E (VE) on this injury. Methods Experimental control group, soman-induced model group and VA and VE experimental group. Before soman exposure, the treated rapeseed oil (1 mL · kg-1 · d-1) in the control group and the soman-induced model group, the VA and VE experimental groups were treated with VA2 mg · kg-1 and VE2 · 5 mg · Kg-1 dose ig, a total of 9d. d 10 Except for the control group, all the other rats were challenged with scorpion abdomen sc (6 3 μg · kg -1) for 2 h and then sacrificed for blood and liver tissues. Changes in serum and liver tissue were observed. The results showed that AChE was significantly decreased in model group, MDA content in serum and liver tissue was significantly increased, SOD activity in serum and liver tissue was decreased, and GSH Px activity in whole blood and liver tissue was significantly decreased. Serum MDA levels decreased in VA and VE experimental groups, and SOD and GSH Px activities in serum and liver increased in both serum and liver tissue. Conclusion Soman exposure caused a serious decrease of AChE activity accompanied by severe free radical damage. Pretreatment of rats with VA and VEig could protect AChE activity, while VA and VE as antioxidants could protect soman Caused by free radical damage