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为了观察ｒａｓ癌基因产物ｒａｓｐ２１在子宫内膜癌及癌前病变组织中的表达，及ｒａｓｐ２１与ＤＮＡ倍体的关系，应用流式细胞术和细胞免疫荧光技术检测了３２例子宫内膜癌、２６例癌前病变和１０例正常内膜组织中的ｒａｓｐ２１的表达情况。结果为正常内膜组织中ｒａｓｐ２１表达阴性，子宫内膜癌ｒａｓｐ２１的表达高于癌前病变。ｒａｓｐ２１表达的荧光指数随子宫内膜癌组织学分级的升高而增加，但与临床分期、肌层浸润和淋巴结转移无关。ＤＮＡ倍体与ｒａｓｐ２１的表达量有关。表明ｒａｓｐ２１在子宫内膜癌和癌前病变有较高的表达，这可能与子宫内膜癌的发生有关。检测ｐ２１蛋白和ＤＮＡ倍体有助于判断肿瘤的恶性程度和预后。
In order to observe the expression of ras oncogene product rasp21 in endometrial carcinoma and precancerous lesions, and the relationship between rasp21 and DNA ploidy, 32 cases of endometrial carcinoma were detected by flow cytometry and cell immunofluorescence technique. The expression of rasp21 in precancerous lesions and 10 normal intimal tissues. The results showed that the expression of rasp21 was negative in normal intima tissue, and the expression of rasp21 in endometrial carcinoma was higher than that in precancerous lesion. The fluorescence index of rasp21 expression increased with the increase of the histological grade of endometrial cancer, but was not related to clinical stage, myometrial invasion and lymph node metastasis. DNA ploidy is related to the expression of rasp21. This indicates that rasp21 is highly expressed in endometrial cancer and precancerous lesions, which may be related to the occurrence of endometrial cancer. Detection of p21 protein and DNA ploidy can help determine the degree of malignancy and prognosis of the tumor.