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目的： 探讨自身抗原Ｒｏ／ＳＳＡ（Ｍｒ＝ ６０ ０００）的抗原决定簇，为自身免疫性疾病机制的研究提供依据。方法： 根据计算机软件进行的蛋白质序列结构分析，采用ＰＣＲ法克隆自身抗原Ｒｏ／ＳＳＡ（Ｍｒ＝ ６０ ０００）多肽片段的ｃＤＮＡ，定向插入表达载体ＰＧＥＸ－２Ｔ，并且导入大肠杆菌中表达重组融合蛋白，用ＧＳＴ亲和层析柱进行纯化，经免疫印迹法与患者阳性血清进行反应。结果： Ｒｏ／ＳＳＡ（Ｍｒ＝ ６０ ０００）的抗原优势决定簇主要存在于１００～１９０位和１９１～３００位，不同患者、不同病程抗原优势决定簇有所不同。结论：抗原驱动机制在自身免疫性疾病的发病中起重要作用。
Objective: To explore the antigenic determinants of autoantigen Ro / SSA (Mr = 60 000), and provide basis for the study of the mechanism of autoimmune diseases. Methods: The cDNA of Ro / SSA (Mr = 60 000) was cloned by PCR using the computer software and inserted into the expression vector PGEX-2T. The recombinant protein was introduced into E. coli to express the recombinant fusion protein. Purified by GST affinity chromatography and reacted with positive sera of patients by Western blotting. Results: The predominant epitopes of Ro / SSA (Mr = 60 000) predominate in the range of 100-190 and 191-300. In different patients, the antigen dominant determinants of different course of disease are different. Conclusion: Antigen-driven mechanism plays an important role in the pathogenesis of autoimmune diseases.