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使用流式细胞仪（ＦＣＭ）动态观察吗啡依赖小鼠在摄入吗啡成瘾期间及吗啡消除后中枢和外周血Ｔ淋巴细胞亚群的变化情况。结果表明：①摄入吗啡后２４ｈ始，胸腺中ＣＤ４和ＣＤ８Ｔ淋巴细胞降低，血液中ＣＤ４降低，ＣＤ８升高，两者比值倒置，连续摄药至ｄ５时最甚。②停止摄入吗啡７２ｈ后，胸腺重量增高，胸腺中ＣＤ４和ＣＤ８Ｔ淋巴细胞即恢复至正常；但吗啡消除１ｗｋ后，血液中的ＣＤ４和ＣＤ８Ｔ淋巴细胞始终未恢复正常。上述结果提示中枢和外周的淋巴细胞亚群对吗啡的反应敏感性存在差异，还提示吗啡在体内对中枢未分化成熟Ｔ淋巴细胞的可逆性损伤或抑制作用和对成熟Ｔ细胞的损伤或抑制更为严重，且有不可逆的作用
Flow cytometry (FCM) was used to dynamically observe the changes of T-lymphocyte subsets in central and peripheral blood of morphine-dependent mice during morphine addiction and morphine abatement. The results showed that: ①24h after morphine ingestion, CD4 and CD8T lymphocytes in the thymus decreased, CD4 in the blood decreased and CD8 increased. ② After 72 hours of ingestion of morphine, the thymus weight increased, and the CD4 and CD8T lymphocytes in the thymus returned to normal. However, after 1wk morphine elimination, the CD4 and CD8T lymphocytes in the blood never returned to normal. These results suggest that central and peripheral lymphocyte subsets response to morphine sensitivity differences exist, but also prompted the morphine in vivo central undifferentiated mature T lymphocytes reversible damage or inhibition and the damage or inhibition of mature T cells more Serious and irreversible