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目的:研究树突状细胞能否转化为泡沫细胞及其在高脂饮食所致兔动脉粥样硬化硬化病变中的分布。方法:建立高胆固醇饮食致新西兰兔动脉粥样硬化模型,取主动脉进行免疫组化和电镜观察,S-100抗体染色用于鉴定树突状细胞来源的泡沫细胞,电镜用于观察其超微结构特点。结果:免疫组化染色观察发现,在正常动脉内膜中,树突状细胞分布极少;在动脉粥样硬化病变中,树突状细胞主要分布在内膜的泡沫细胞富集区域,部分表现出泡沫细胞的形态学特征。电镜观察发现部分树突状细胞胞浆内见大量脂滴沉积,呈现典型的泡沫细胞特点。结论:树突状细胞是动脉粥样硬化中泡沫细胞的一个新来源,这在动脉粥样硬化中可能具有重要的作用。
OBJECTIVE: To study whether dendritic cells can be transformed into foam cells and their distribution in atherosclerotic lesions in rabbits induced by high-fat diet. Methods: The model of atherosclerosis induced by hypercholesterolemia in rabbits was established. The aorta was taken for immunohistochemistry and electron microscopy. S-100 antibody staining was used to identify the dendritic cell-derived foam cells. Electron microscopy was used to observe the ultrastructure of dendritic cells Structural features. Results: Immunohistochemical staining showed that there were few dendritic cells in normal intima. In atherosclerotic lesions, dendritic cells were mainly distributed in the foam cell-rich region of the intima, The morphological characteristics of the foam cells. Electron microscopy showed that a large amount of lipid droplets deposited in the cytoplasm of some dendritic cells, showing typical characteristics of foam cells. Conclusion: Dendritic cells are a new source of foam cells in atherosclerosis and may play an important role in atherosclerosis.