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目的 :研究常用化疗药物对 SGC- 790 1人胃癌细胞株端粒酶活性的调节作用 ,探讨端粒酶作为化疗敏感性指标的可行性。方法 :选择顺铂、丝裂霉素 C、阿霉素及 5 -氟尿嘧啶和甲酰四氢叶酸处理胃癌细胞 ,采用半定量 TRAP-银染法检测药物处理前后的端粒酶活性 ,流式细胞学检测细胞周期和细胞凋亡率。结果 :顺铂、丝裂霉素C和阿霉素在诱导 SGC- 790 1细胞凋亡的同时 ,下调端粒酶活性 ,且抑制作用呈时间依赖关系 ,5 -氟尿嘧啶和甲酰四氢叶酸对端粒酶活性无明显抑制作用。结论 :观测细胞株端粒酶的活性变化可作为化疗药物敏感性的指标 ,可合理选择新的化疗药物。
Objective: To study the regulation of telomerase activity in SGC-7901 human gastric cancer cell lines by commonly used chemotherapeutic drugs, and to explore the feasibility of using telomerase as an indicator of chemosensitivity. METHODS: Gastric cancer cells were treated with cisplatin, mitomycin C, adriamycin, 5-fluorouracil and leucovorin. Semiquantitative TRAP-silver staining was used to detect telomerase activity before and after drug treatment. Flow cytometry The cell cycle and apoptosis rate were measured. RESULTS: Cisplatin, mitomycin C, and adriamycin induced apoptosis of SGC-7901 cells while down-regulating telomerase activity in a time-dependent manner. 5-Fluorouracil and leucovorin Telomerase activity was not significantly inhibited. Conclusion: Observing the change of telomerase activity in cell lines can be used as an indicator of the sensitivity of chemotherapy drugs, and new chemotherapeutic drugs can be reasonably selected.