论文部分内容阅读
目的以大鼠为动物模型研究了海参皂苷单体Echinoside A(EA)在体内的消化吸收特性。方法Wistar大鼠适应饲养1周后,按体质量分为10组,每组3只。禁食不禁水12h后,给大鼠分别按照30mg/kg·BW灌胃配置好的海参皂苷EA溶液1mL,分别在灌胃后0、0.5、1、2、3、4、7、9、11、24h取血,建立并优化血清中海参皂苷EA的HPLC-MS检测方法,检测不同时间节点大鼠血中EA含量。结果实验结果显示,海参皂苷单体EA液质检测的定量限(LOQ)为2.12ng,在0.3~20μg/mL的浓度范围内线性良好(R2=0.994 6)。海参皂苷EA灌胃后在血中迅速出现,在3h出现最高峰值,浓度达到0.83μg/mL,之后在7h出现另1个较小峰,9h后恢复至4h水平(约0.24μg/mL),直至24h。结论本文建立了血清中海参皂苷EA的液相色谱-质谱联用检测方法,发现海参皂苷单体EA可经消化道吸收,该结果为海参皂苷在保健食品及药物中的应用提供了科学依据。
OBJECTIVE: To study the digestion and absorption characteristics of Echinoside A (EA) in vivo by using rat as an animal model. Methods Wistar rats were housed for 1 week and divided into 10 groups according to their body weight, with 3 rats in each group. Fasting can not help but water 12h, respectively, according to the rats were 30mg / kg · BW gavage configured ginseng saponin EA solution 1mL, respectively, after gavage 0,0.5,1,2,3,4,7,9,11 , And blood was drawn for 24h to establish and optimize the HPLC-MS method for the determination of serum ginsenoside EA. The levels of EA in the blood of rats at different time points were detected. Results The experimental results showed that the limit of quantification (LOQ) of liquid components of sea cucumber Saponin monomer EA was 2.12ng, and the linearity was good (R2 = 0.994 6) in the range of 0.3 ~ 20μg / mL. Ginsenosides EA appeared rapidly in the blood after gavage, with the highest peak at 3h, the concentration reached 0.83μg / mL, followed by another one at 7h, returned to 4h after 9h (about 0.24μg / mL) Until 24h. Conclusion The method of liquid chromatography-mass spectrometry for the determination of ginsenosides EA in serum was established in this paper. It was found that the ginseng saponin EA could be absorbed through the digestive tract. This result provides a scientific basis for the application of ginsenosides in health food and medicine.