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室内生物测定是植物对除草剂等化学物质耐受性鉴定的一种常用筛选方法,已广泛应用于大豆、稗草、棉花等植物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但麦草畏的室内生物测定方法和大豆对麦草畏耐受性相关研究尚未见报道。本研究以麦草畏对催芽大豆下胚轴伸长抑制率为评价指标,结合回归方程曲线分析和抑制中浓度分析,建立了大豆对麦草畏耐受性室内生物测定方法,确定以300μg/L麦草畏筛选浓度作为大豆室内生物测定临界筛选浓度。利用该方法对35份源自微核心种质的大豆品种进行鉴定,结果表明,随麦草畏浓度增加,不同品种对麦草畏的耐受性存在显著差异,大豆品种对麦草畏的耐受性降低,从中筛选出对麦草畏耐受性较高的大黄豆-1和什邡螺丝豆。本研究结果为培育抗麦草畏品种的亲本选配以及后代选择提供了理论依据、材料和技术支撑。
Indoor bioassay is a common screening method for the tolerance of plants to chemicals such as herbicides. It has been widely used in the tolerance of herbicides such as glyphosate and chlorimuron to plants such as soybean, barnyard grass and cotton However, studies on dicamba indoor bioassays and soybean on dicamba resistance have not been reported yet. In this study, dicamba on germination of hypocotyl elongation inhibition rate for the evaluation index, combined regression curve regression analysis and inhibition of mid-concentration analysis, the establishment of soybean dicamba tolerance indoor bioassay method to determine the 300μg / L wheat straw Fear screening concentration as indoor screening bioassay critical concentration. The results showed that the tolerance of different cultivars to dicamba was significantly different with the increase of dicamba concentration, and that of soybean cultivars was lower than that of dicamba , Which screened large dicamba-1 and Shifang screw beans tolerant of dicamba. The results of this study provide the theoretical basis, material and technical support for the selection of parents and selection of offspring for cultivating dicamba.