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目的:验证巩堤丸提高机体免疫力的同时,兼具抑菌的主要药效学作用。方法:①通过打孔法研究巩堤丸的体外抑菌作用。②巩堤丸方对正常小鼠炭廓清功能的影响:试验选用ICR小鼠50只,雄性,随机分为正常组,六味地黄丸组(剂量2g.kg-1),巩堤丸方的高、中、低剂量(即18,9,4.5 g.kg-1)组。③巩堤丸方对正常小鼠迟发型超敏反应的影响,选用50只ICR种小鼠,雄性,体重18～22 g,随机分为5组,每组10只,分组及用药剂量同上。结果:巩堤丸除对金黄色葡萄球菌、变形杆菌无抑制作用外,对乙型溶血性链球菌及大肠埃希菌均有一定的体外抑菌作用,但与西药组相比,在抑菌方面劣于西药组。与正常对照组相比较,巩堤丸方对正常小鼠炭廓清功能有明显促进作用,低剂量时最显著(P<0.01),提示该药能够增加小鼠非特异免疫功能。与正常对照组相比较,巩堤丸方低剂量组对DNCB诱导小鼠迟发型超敏反应有显著的增强作用(P<0.05)。结论:巩堤丸方具有抑菌、提高免疫力等的综合作用,从而对于泌尿系感染(劳淋)属于脾肾阳虚,应用西药治疗不彻底造成泌尿系感染慢性化的患者具有良好的治疗作用,可用于慢性泌尿系感染缓解期的治疗。
Objective: To verify Gongdi pill to improve immunity, while the main antimicrobial pharmacodynamic effect. Methods: ① study the antibacterial effect of Gongdian pill in vitro by perforating method. (2) The effect of Gongdianfang on carbon clearance of normal mice: Fifty ICR mice were randomly divided into normal group, Liuweidihuangwan group (dose 2g.kg-1) and high , Medium and low dose (ie 18,9,4.5 g.kg-1) group. ③ The effect of Gongdianfang on the delayed-type hypersensitivity in normal mice. Fifty ICR mice were selected and males, weighing 18-22 g, were randomly divided into 5 groups with 10 in each group. The doses and doses were the same as above. Results: In addition to the embankment pill Staphylococcus aureus, Proteus no inhibitory effect on both hemolytic streptococcus and Escherichia coli have some antibacterial effect, but compared with Western medicine group, in the inhibition of bacteria Aspects inferior to Western medicine group. Compared with the normal control group, Gongtiao pill can significantly promote the carbon clearance function of normal mice, and the most significant effect at low dosage (P <0.01), suggesting that the drug can increase non-specific immune function in mice. Compared with the normal control group, low dose group of Gongtianshufang significantly enhanced the delayed-type hypersensitivity reaction induced by DNCB in mice (P <0.05). Conclusion: Gongtiao Pill has the combined effect of inhibiting bacteria and improving immunity, so it has a good treatment for patients with urinary tract infection (LOREN) which belongs to spleen and kidney yang deficiency and who is not thoroughly treated with western medicine and causes chronic urinary tract infection Role, can be used for the treatment of chronic urinary tract infection remission.