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目的:探讨侧脑室注射obestatin对大鼠血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1水平的影响以及对胃排空的调控。方法:侧脑室注射obestatin,采用酶免疫测定(EIA)法检测血浆酰基化ghrelin、去酰基化ghrelin、nesfatin-1水平以及胃排空率的变化。结果:侧脑室分别注射0.1、0.3或1.0 nmol obestatin,大鼠血浆酰基化ghrelin、去酰基化ghrelin以及nesfatin-1水平无显著改变(P>0.05),且酰基化ghrelin与去酰基化ghrelin比率无显著改变(P>0.05);侧脑室注射obestatin,大鼠摄食量无显著改变,但胃排空率明显增加(P<0.05);胃排空率明显延迟(P<0.05)。与侧脑室注射1.0 nmol Obestatin组相比,注射1.0 nmol Obestatin+CRF,大鼠摄食量无显著改变,胃排空率明显延迟(P<0.05)。各组摄食量及进入十二指肠内食物量无明显差异(P>0.05)。结论:中枢obestatin促进大鼠的胃排空,可能与h/r CRF通路有关。
Objective: To investigate the effect of intracerebroventricular injection of obestatin on the plasma levels of acyl ghrelin, deacylated ghrelin and nesfatin-1 in rats and the regulation of gastric emptying. Methods: Obestatin was injected into the lateral ventricle. The levels of acylated ghrelin, deacylated ghrelin, nesfatin-1 and gastric emptying rate were measured by enzyme immunoassay (EIA). Results: 0.1, 0.3 or 1.0 nmol obestatin was injected into the lateral ventricle respectively. There was no significant change in the plasma levels of acylated ghrelin, deacylated ghrelin and nesfatin-1 (P> 0.05), and the ratio of acylated ghrelin to deacylated ghrelin (P> 0.05). There was no significant change in the food intake of obestatin in the lateral ventricle but the gastric emptying rate was significantly increased (P <0.05). The gastric emptying rate was significantly delayed (P <0.05). Compared with intracerebroventricular injection of 1.0 nmol Obestatin, there was no significant change in food intake and delayed gastric emptying (P <0.05) after 1.0 nmol Obestatin + CRF injection. There was no significant difference in food intake and food intake in each group (P> 0.05). Conclusion: Central obestatin can promote gastric emptying in rats, which may be related to h / r CRF pathway.