目前,圆环病毒(circovirus)是已知能够感染动物的最小的DNA病毒之一.在本研究中,通过高通量测序分析了采集自中国内蒙古绵羊和骆驼体表寄生的亚洲璃眼蜱(H yalomma asiaticum)和草原革蜱(Dermacentor nuttalli),检测在这2种蜱虫中是否存在圆环病毒,并通过反向半巢式PCR扩增得到了1个拥有2042个碱基对的环状单链DNA(ssDNA)完整基因组序列.进一步分析表明,该环状基因组有2个主要的开放阅读框(ORF),推定其分别编码复制酶(Rep)和衣壳蛋白(Cap).序列分析和系统发育分析表明,该圆环病毒属于鸽圆环病毒.综合分析表明,内蒙古地区蜱虫中携带有鸽圆环病毒,该结果为深入探索鸽圆环病毒在内蒙古地区的流行情况以及内蒙古地区蜱虫携带病原体积累了数据.“,”Circovirus is one of the smallest known DNA viruses known to be able to infect animals.In this report,circovirus was detected by high-throughput sequencing in two tick species,Hyalom-ma asiaticum and Dermacentor nuttalli collected from sheep and camels in Inner Mongolia,China.A complete genomic sequence of 2042 base pairs of cyclic single-stranded DNA(ssDNA) was ob-tained by reverse semi-nested PCR amplification.Evolutionary analysis further revealed that it be-longed to circovirus with two major open read frames(ORFs) encoding replicase and capsid pro-teins,respectively.Sequence and phylogenetic analysis suggested that the circovirus belonged to pi-geon circovirus.The results demonstrated the presence of pigeon circovirus in ticks in Inner Mon-golia,which provide the basis for future exploration of the prevalence of pigeon circovirus in Inner Mongolia and the pathogens carried by ticks in Inner Mongolia.