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目的初步阐明奥曲肽（Ｏｃｔｒｅｏｔｉｄｅ，ＳＭＳ２０１，９９５，商品名：善得定）与乳腺肿瘤细胞凋亡的关系。方法取手术切除的１６例女性乳腺癌标本，制备肿瘤单细胞悬液，加入善得定（０．１μｇ／ｍｌ）和三苯氧胺（１μｇ／ｍｌ），用ＤＮＡ断端标记法（ＴＤＴ法）分析药物及ＥＲ、ＰＲ对乳腺肿瘤细胞凋亡的影响。结果加入善得定４小时后凋亡率为１０．６±６．９％，１８小时为１４．３±８．８％，ＥＲ（＋）ＰＲ（＋）组４小时善得定凋亡率为１４．３±５．７％，１８小时为２０．２±７．１％，加用三苯氧胺后１８小时组凋亡率为２６．３±８．９％，而ＥＲ（－）ＰＲ（－）组４小时善得定组凋亡率为４．４±３．０％，１８小时为６．５±４．０％，加用三苯氧胺后１８小时组凋亡率为８．８±４．１％，与ＥＲ（＋）ＰＲ（＋）组相比有明显差异（Ｐ＜０．０５）。结论善得定可诱导乳腺肿瘤细胞凋亡，且短时间内即可达到一定的凋亡率，善得定对ＥＲ（＋）ＰＲ（＋）的乳腺肿瘤细胞较敏感，而对ＥＲ（－）ＰＲ（－）组则基本无影响，善得定加用三苯氧胺的治疗有望成为乳腺癌患者术后一个新的辅助生物治疗手段
Objective To clarify the relationship between Octreotide (SMS201,995, trade name: Sandostine) and apoptosis of breast tumor cells. METHODS: Sixteen female breast cancer specimens surgically resected were used to prepare single cell tumor suspensions. Shanduzing (0.1 μg/ml) and tamoxifen (1 μg/ml) were added. The drug was analyzed by DNA fragmentation (TDT). The effects of ER and PR on apoptosis of breast tumor cells. Results The apoptotic rate was 10.6±6.9% at 4 hours after adding Shandisein and 14.3±8.8% at 18 hours, and the rate of azepine at 4 hours in ER(+)PR(+) group was significant. It was 14.3±5.7%, and it was 20.2±7.1% at 18 hours. After 18 hours of adding tamoxifen, the apoptotic rate was 26.3±8.9%, while ER(-)PR(- The apoptotic rate was 4.4±3.0% at 4 hours of Shanduzing group, 6.5±4.0% at 18 hours, and 8.8±4 at 18 hours after adding tamoxifen. 1% was significantly different from the ER(+)PR(+) group (P<0.05). Conclusion Shanduzing can induce apoptosis of breast tumor cells, and it can reach a certain rate of apoptosis in a short time. Shandinging is more sensitive to ER (+) PR (+) breast tumor cells, but to ER (-) The PR(-) group had no effect, and the treatment with satendine plus tamoxifen was expected to become a new adjuvant biologic therapy for postoperative breast cancer patients.