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目的通过分析人乳腺癌COX-2mRNA表达与雌激素受体水平的相关性,以探讨COX-2在人乳腺癌发病机制中的作用。方法选择17例浸润性乳腺癌患者冰冻癌组织,采用定量RT-PCR方法癌组织中COX-2mRNA水平。用配体结合方法测定雌二醇受体(ER)及孕酮受体(PR)水平。结果COX-2mRNA表达与PR呈正相关(P<0.05)。结论人乳腺癌患者COX-2mRNA表达与PR水平呈正相关,PR和COX-2mRNA测定有助于选择治疗方案和判定预后。
Objective To investigate the role of COX-2 in the pathogenesis of human breast cancer by analyzing the correlation between COX-2 mRNA expression and estrogen receptor level in human breast cancer. Methods Seventeen patients with invasive breast cancer were enrolled in this study. The levels of COX-2 mRNA in cancer tissue were determined by quantitative RT-PCR. The levels of estradiol receptor (ER) and progesterone receptor (PR) were determined by ligand binding assay. Results COX-2 mRNA expression was positively correlated with PR (P <0.05). Conclusion The expression of COX-2mRNA in human breast cancer patients is positively correlated with the level of PR. The determination of PR and COX-2mRNA may be helpful in the selection of treatment and prognosis.