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目的探讨血管紧张素转化酶(ACE)和C反应蛋白(CRP)在慢性房颤犬心房组织中的表达及意义。方法随机将健康杂种犬16条分为正常对照组、单纯房颤起搏组各8只。房颤组植入埋藏式高频率心脏起搏器[(500±20)次/min],起搏24周后处死动物,分别于左、右心房取材,采用免疫组织化学法,检测心房组织中血管紧张素转化酶(ACE)、C反应蛋白(CRP)以及血管紧张素转化酶抑制剂(ACEI)的表达变化。正常对照组未植入起搏器,与房颤组同步行相应检查。结果与对照组比较,房颤组心房组织中ACE和CRP表达水平显著增强,而ACEI的表达水平明显下降,各组间比较差异显著(P<0.01)。结论肾素血管紧张素(RAS)系统、炎症与房颤之间可能存在着一定的联系,阻断RAS系统有可能抑制房颤的发生、发展。
Objective To investigate the expression and significance of angiotensin-converting enzyme (ACE) and C-reactive protein (CRP) in atrial fibrillation of dogs with chronic atrial fibrillation. Methods 16 healthy mongrel dogs were randomly divided into normal control group and 8 rats in atrial fibrillation pacing group. Atrial fibrillation group implanted buried high-frequency pacemaker [(500 ± 20) times / min], pacing 24 weeks after the animals were sacrificed, respectively, the left and right atrium drawn, using immunohistochemistry to detect atrial tissue Angiotensin converting enzyme (ACE), C-reactive protein (CRP) and angiotensin-converting enzyme inhibitor (ACEI) expression changes. The normal control group was not implanted pacemaker, with the same line with AF group corresponding examination. Results Compared with the control group, the expression of ACE and CRP in atrial fibrillation group was significantly increased, while the expression of ACEI was significantly decreased (P <0.01). Conclusions There may be a relationship between renin angiotensin system (RAS), inflammation and atrial fibrillation. Blockade of RAS system may inhibit the occurrence and development of atrial fibrillation.