论文部分内容阅读
[目的]研究深圳市HIV-1感染孕产妇淋巴细胞亚群变化和毒株亚型分布特征。[方法]通过流式细胞仪分析HIV-1感染孕产妇CD4/8/3细胞、B淋巴细胞、NK细胞亚群和CD45阳性白细胞绝对计数值,比较这些指标与其他女性HIV-1携带者的差异;RT-PCR套式扩增深圳地区HIV-1感染孕产妇HIV-1病毒env区基因,经基因测序后构建基因进化树并分析其亚型分布。[结果]发现HIV-1感染孕产妇CD4/8/3细胞、B淋巴细胞、NK细胞亚群和CD45阳性白细胞绝对计数值均低于其他女性HIV-1携带者;HIV-1感染孕产妇HIV-1毒株主要为01_AE型。[结论]HIV-1感染孕产妇免疫功能偏低,加强该人群的免疫功能监测,避免继发感染,对降低婴儿HIV-1感染率有重要作用。
[Objective] To study the changes of lymphocyte subsets and the subtypes distribution of HIV-1 infection in Shenzhen. [Methods] The absolute counts of CD4 / 8/3, B lymphocyte, NK cell subgroup and CD45 positive leucocyte in HIV-1 infected pregnant women were analyzed by flow cytometry. The correlation between these indexes and other female HIV-1 carriers RT-PCR was used to amplify the HIV-1 HIV-1 env region gene of HIV-1 infection in Shenzhen. The gene phylogenetic tree was constructed and the subtype distribution was analyzed. [Results] The absolute counts of CD4 / 8/3 cells, B lymphocytes, NK cell subsets and CD45 positive leukocytes in HIV-1 infected pregnant women were found to be lower than those of other HIV-1 carriers. HIV- -1 strain mainly 01_AE type. [Conclusion] The immune function of pregnant women with HIV-1 infection is low. Monitoring the immune function of this population and avoiding secondary infection have an important role in reducing the HIV-1 infection rate in infants.