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水浴加热后的盐酸土霉素在磷酸介质中，过氧化氢存在下与高碘酸钠反应产生强烈的化学发光。结合流动注射技术建立了一种测定盐酸土霉素的化学发光新方法。方法的线性范围为１．０ｘ１０－８～６．０ｘ１０－６ｇ／ｍＬ盐酸土霉素。检出限为４．３ｘ１０－９ｇ／ｍＬ，ＲＳＤ为２．５％（Ｃｓ＝２．０ ｘ １０－６ｇ／ｍＬ；ｎ＝１１）。
Oxytetracycline hydrochloride heated in a water bath is reacted with sodium periodate in the presence of hydrogen peroxide in a phosphoric acid medium to produce intense chemiluminescence. A new chemiluminescence method for the determination of oxytetracycline hydrochloride was established by flow injection technique. The linear range of the method is 1.0 × 10 -8 ~ 6.0 × 10 -6 g / mL oxytetracycline hydrochloride. The limit of detection was 4.3x10-9 g / mL and RSD was 2.5% (Cs = 2.0 x 10-6 g / mL; n = 11).