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目的观察1.8GHz[比吸收率(SAR)为3Wkg]微波(MW)对4种化学诱变剂[丝裂霉素C(MMC)、博莱霉素(BLM)、4硝基喹啉氧化物(4NQO)、甲基甲烷磺酸酯(MMS)]诱发的人外周血淋巴细胞DNA损伤的影响。方法采用彗星试验体外实验分别在暴露后0h和21h检测1.8GHz微波、4种化学诱变剂及微波联合4种诱变剂所诱发的人淋巴细胞DNA损伤。所用的指标为尾长(TL)和尾相(TM)。微波辐射和化学诱变剂暴露时间分别为2h和3h。结果微波组所诱发的DNA损伤与对照组相比,差异无统计学意义(P>0.05);微波分别与MMC和4NQO的联合暴露组所诱发的DNA损伤明显高于相应浓度的MMC组和4NQO组,差异有统计学意义(P<0.01或P<0.05);但微波对BLM和MMS所诱发DNA损伤的增强效应不明显,差异无统计学意义(P>0.05)。结论1.8GHz(SAR为3Wkg)微波暴露2h并不诱发人淋巴细胞DNA损伤,但能增强MMC、4NQO的DNA损伤效应。
OBJECTIVE To observe the effects of 1.8GHz microwave absorption rate (MW) on four chemical mutagens [mitomycin C (MMC), bleomycin (BLM), 4-nitroquinoline oxide (4NQO), methyl methanesulfonate (MMS)] induced DNA damage in human peripheral blood lymphocytes. Methods The DNA damage of human lymphocytes induced by 1.8GHz microwave, 4 kinds of chemical mutagens and 4 kinds of microwave mutagens were detected by comet assay in vitro at 0h and 21h respectively. The indicators used are tail length (TL) and tail phase (TM). Exposure time of microwave irradiation and chemical mutagens were 2h and 3h respectively. Results Compared with the control group, the DNA damage induced by microwave group had no significant difference (P> 0.05). The DNA damage induced by microwave combined with MMC and 4NQO group was significantly higher than that of MMC group and 4NQO Group, the difference was statistically significant (P <0.01 or P <0.05). However, the enhancement effect of microwave on DNA damage induced by BLM and MMS was not significant, the difference was not statistically significant (P> 0.05). Conclusion Microwave exposure at 1.8GHz (SAR 3Wkg) for 2h did not induce DNA damage in human lymphocytes, but enhanced the DNA damage effect of MMC and 4NQO.