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用插入LacZ基因的重组病毒株建立BALB/c小鼠巨细胞病毒 (murinecytomegalovirus ,mCMV)性肝炎模型。20只BALB/c甲基强的松龙免疫抑制小鼠 ,腹腔接种1×106PFU病毒悬液 ,动态观察血单个核细胞和组织中mCMV感染及肝组织病理变化。结果 :①mCMV感染小鼠血单个核细胞中X -gal染色阳性细胞在48h时开始出现 ,第3天有所减少 ,在第6天达到高峰;②肝、肺、肾组织病毒感染72h后可见 ,在第12天斑点数最多 ,以肝组织病毒感染多见;③肝组织病变在病毒感染后96h开始出现 ,在第12天达到高峰。提示 :利用重组mCMV建立的mCMV性肝炎小鼠模型 ,在感染后2周仍保持较高感染水平 ,为CMV的研究提供了一个良好的实验模型。
BALB / c mouse model of murine cytomegalovirus (mCMV) hepatitis was established by using recombinant virus with LacZ inserted. Twenty BALB / c methylprednisolone immunosuppressed mice were inoculated intraperitoneally with 1 × 106 PFU of virus suspension to observe the infection of mCMV in blood mononuclear cells and tissues and the pathological changes of liver tissue. Results: (1) X-gal positive cells in blood mononuclear cells of mice infected with mCMV began to appear at 48h, decreased on the third day and reached the peak on the sixth day; (2) After 72h infection of liver, lung and kidney tissues, In the 12th day the spot number was the most, which was more common in liver tissue virus infection. ③ The liver tissue lesion appeared at 96h after virus infection and peaked on the 12th day. It is suggested that the mCMV hepatitis mouse model established by recombinant mCMV maintained a high level of infection two weeks after infection, which provided a good experimental model for the study of CMV.