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制备了掺杂二茂铁甲醇(Fc-OH)的丝网印刷电极,以Fc-OH作为氧化剂,研究了尿酸(UA)在丝网印刷电极的电化学氧化行为,建立了一种新的用于检测UA的方法。方法无需使用尿酸氧化酶,且能提高传感器的灵敏度,并且利用抗坏血酸氧化酶催化抗坏血酸(AA)的氧化来排除血液中AA的影响。计时电流法(I-t)测试0.3V处的氧化峰电流与UA浓度在10μmol/L至1.5 mmol/L的范围内呈较好的线性关系,检出限为5μmol/L。电极制作简单,重现性好,能有效的排除AA、对乙酰氨基酚(ACP)等的干扰,方法已成功应用于实际样品中UA的检测。
A novel Fe-Fe-based screen printed electrode was prepared. The electrochemical oxidation behavior of uric acid (UA) on the screen printed electrode was studied by using Fc-OH as oxidant. In the detection of UA method. The method eliminates the need for uric acid oxidase and increases the sensitivity of the sensor, and uses the ascorbate oxidase to catalyze the oxidation of ascorbic acid (AA) to eliminate the effects of AA in the blood. Chromogenic current (I-t) test 0.3V at the peak current and UA concentration in the range of 10μmol / L to 1.5 mmol / L showed a good linear relationship with a detection limit of 5μmol / L. The electrode is simple and reproducible, and can effectively eliminate the interference of AA, acetaminophen (ACP) and the like. The method has been successfully applied to the detection of UA in real samples.