论文部分内容阅读
本文报告了在采用“PH自动控制系统”控制培养液pH值的条件下,用微电极测定兔甲状腺切片腺泡细胞膜电位与泡腔电位的氏液p0.02,氧饱和)表面灌流下,0～3h内细胞膜电位相对稳定,平均为—33.31.6mv(n=19);～4h膜电位趋于去极化,～5h膜电位较～1h明显去极化、p<0.05),但至25～27h膜电位仍可维特在—22.7±2.0mv(n=7),腺泡腔电位始终没有明显变化,0～27h内平均为—12.4±0.4mv,(n=38)。与其他作者结果比较,我们认为在平衡盐溶液中甲状腺细胞膜电位可能为—42mv左右,腺泡腔电位为—12mv左右;本实验条件优于国外采用5％CO_2混合气控制pH的条件。
This paper reports the use of “PH automatic control system” to control the pH of the culture medium conditions, the use of microelectrodes to measure the thyroid gland acinar cell membrane potentials and pouch potentials of the solution p0.02, oxygen saturation) surface perfusion, 0 The cell membrane potential was relatively stable within ~ 3 h with an average of -33.31.6 mv (n = 19); the membrane potential tended to depolarize at ~ 4 h, and the membrane potential at ~ 5 h was significantly depolarized at ~ 1 h (p <0.05) The membrane potentials at ~ 27h were still significantly lower at -22.7 ± 2.0mv (n = 7). There was no significant change in acinar potential at all, with a mean of -12.4 ± 0.4mv within 0-27h (n = 38). Compared with the results of other authors, we think that in the balanced salt solution, the thyroid cell membrane potential may be about -42mv, and the acinar potential is about -12mv. The experimental conditions are better than the foreign control of pH by 5% CO_2 mixture.