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目的:研究以HPLC法同时测定中药退黄外洗液中栀子苷、甘草苷、柚皮苷含量的方法。方法:采用高效液相色谱法。色谱柱Agilent SB-C18柱(4.6mm×250mm,5μm),流动相以甲醇-水(40∶60),流速1.0mL·min-1,检测波长,λ1238nm,λ2283nm,柱温30℃。结果:栀子苷在5.1～127.5ng线性关系良好(r=0.9997),平均回收率为99.41%,RSD0.24%(n=6);甘草苷在2.3～53ng线性关系良好(r=0.9995),平均回收率为98.49%,RSD1.23%(n=6);柚皮苷在4.9～123ng线性关系良好(r=0.9999),平均回收率为99.41%,RSD0.48%(n=6)。阴性对照无干扰。结论:该法操作简便,结果准确可靠,可作为中药退黄外洗液的质量控制方法。
OBJECTIVE: To study the simultaneous determination of geniposide, liquiritin, naringin in traditional Chinese medicine dandruff lotion by HPLC. Methods: Using high performance liquid chromatography. The mobile phase consisted of methanol-water (40:60), flow rate 1.0 mL · min-1, detection wavelength, λ1238 nm, λ2283 nm and column temperature 30 ℃. The column was equipped with an Agilent SB-C18 column (4.6 mm × 250 mm, 5 μm). Results: The calibration curve of geniposide was linear in the range of 5.1 ~ 127.5 ng (r = 0.9997), with an average recovery of 99.41% and a RSD of 0.24% (n = 6) , The average recovery was 98.49% and the RSD was 1.23% (n = 6). The naringin showed a good linearity at 4.9-123 ng (r = 0.9999) with an average recovery of 99.41% and RSD 0.48% . Negative control without interference. Conclusion: The method is simple, accurate and reliable, and can be used as a quality control method for Chinese traditional medicine.