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背景HPA-5b是最常见的妊娠诱导的血小板特异性抗体。由抗HPA-5b所导致的新生儿同种免疫性血小板减少症很少引起婴儿发生严重的出血,但对受影响的儿童所引发的后遗症则可能非常严重。因此,有必要鉴别新生儿同种免疫性血小板减少症是否有危险发生。研究设计与方法 用血小板抗原的单克隆特异性抗体固相试验检测妊娠诱导的HPA-5b抗体的IgG效价、亚类及轻链成分。血清取自12名母亲,她们的16次妊娠出现了HPA-5b不合胎儿(HPA-5b阳性)。8名母亲产有血小板计数正常的婴儿。有3份母体血清是在分娩出患严重血小板减少症婴儿后所取得的。3名同种免疫的妇女
Background HPA-5b is the most common pregnancy-induced platelet-specific antibody. Neonatal alloimmune thrombocytopenia caused by anti-HPA-5b rarely causes severe bleeding in infants, but sequelae to affected children can be severe. Therefore, it is necessary to identify whether there is a risk of neonatal alloimmune thrombocytopenia. Study Design and Methods IgG titer, subclass, and light chain components of pregnancy-induced HPA-5b antibodies were determined using a monoclonal antibody-specific solid phase assay of platelet antigens. Serum was taken from 12 mothers with HPA-5b antepartum (HPA-5b positive) in 16 pregnancies. Eight mothers had infants with normal platelet counts. Three maternal sera were obtained after giving birth to an infant with severe thrombocytopenia. Three same-sex women