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作者以人乳头状瘤病毒(HPV)16DNA为探针,采用斑点杂交及Southern分子杂交技术检测24例食管癌及其相应癌旁组织中的HPV16DNA。结果:在食管癌组织中,HPV 16DNA斑点及Southern杂交的阳性率为50％(12/24),癌旁组织为37％(9/24);经Bam H I酶切的食管癌及癌旁组织中,杂交后出现7.2kb的HPV16DNA特异性阳性区带;经Pst I酶切杂交后出现2.7kb、2.3kb、1.75kb、1.18kb四条HPV16DNA阳性区带;未经酶切者来发现阳性杂交带出现。提示人乳头状瘤病毒基因组确实存在于食管癌及癌旁组织DNA中,且多以整合状态存在。
Using HPV16 DNA as a probe, we detected the HPV16 DNA in 24 cases of esophageal cancer and its corresponding paracancerous tissues by dot blot hybridization and Southern blot hybridization. Results: In esophageal cancer tissues, the positive rate of HPV 16 DNA spots and Southern hybridization was 50% (12/24) and that of paracancerous tissues was 37% (9/24). The positive rates of esophageal cancer and paracancerous tissues , The positive HPV16DNA positive band of 7.2kb appeared after hybridization; four HPV16DNA positive bands of 2.7kb, 2.3kb, 1.75kb and 1.18kb appeared after Pst I digestion; the positive hybridization bands were found without enzyme digestion appear. Prompted that the human papilloma virus genome does exist in esophageal and paracancerous tissue DNA, and many exist in an integrated state.