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目的了解上海金山地区乙型肝炎病毒(HBV)感染者基因型分布及其与临床的关系。方法401例HBV DNA阳性患者采用聚合酶链反应(PCR)-酶联免疫吸附试验(ELISA)进行HBV DNA定量、微流基因芯片法进行基因分型、ELISA检测e抗原(HBeAg)及肝功能检查后进行综合分析。结果B型198例(49.38%),C型169例(42.14%),B/C混合型20例(4.99%),未分型14例(3.49%),未发现其他基因型。B型在慢性乙型重度肝炎(CSHB)患者中的比例显著低于无症状携带者(ASC)和慢性乙型轻、中度肝炎(CMHB)(P<0.01),而C型在CSHB患者中的比例显著高于ASC和CMHB(P<0.01)。ASC中B型患者的HBV DNA水平最低。C型患者的丙氨酸氨基转移酶(ALT)和γ-谷氨酰基转移酶(GGT)水平均高于B型。结论上海金山地区HBV感染者基因型以B型为主,C型次之,少量B/C混合型,C型与重肝病关系比B型密切。
Objective To investigate the genotype distribution of hepatitis B virus (HBV) infection in Shanghai Jinshan area and its clinical significance. Methods One hundred and four patients with positive HBV DNA were enrolled in this study. HBV DNA was quantified by polymerase chain reaction (PCR) - enzyme - linked immunosorbent assay (ELISA), genotyping was performed by microfluidic microarray, ELISA was used to detect e antigen (HBeAg) and liver function After a comprehensive analysis. Results There were 198 cases (49.38%) in B group, 169 cases (42.14%) in C type, 20 cases (4.99%) in B / C mixed type and 14 cases (3.49%) in unbleached type. No other genotypes were found. The proportion of type B in patients with chronic severe hepatitis B (CSHB) was significantly lower than those in asymptomatic carriers (ASC) and chronic hepatitis B (CMHB) (P <0.01), while in patients with CSHB, type C Was significantly higher than that of ASC and CMHB (P <0.01). Patients with type B have the lowest levels of HBV DNA in ASC. Patients with type C had higher levels of alanine aminotransferase (ALT) and gamma-glutamyltransferase (GGT) than type B. Conclusion The genotypes of HBV infection in Jinshan district of Shanghai are mainly B type, C type followed by a small amount of B / C mixed type, and C type and severe liver disease are more closely related to B type.