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目的:对滁菊叶的化学成分进行研究。方法:通过硅胶、Sephadex LH-20、制备高效液相等方法,对滁菊叶乙醇提取物进行分离纯化,根据理化性质和波谱数据鉴定化合物结构;以DPPH·自由基清除能力检测所得化合物的抗氧化能力。结果:从滁菊叶乙醇提取物中分离鉴定了21个化合物,分别为:二十八烷醇(1)、β-谷甾醇(2)、羽扇豆醇(3)、α-香树脂醇(4)、胡萝卜苷(5)、ineupatorolide B(6)、紫丁香苷(7)、绿原酸(8)、petasiphenol(9)、大黄素甲醚(10)、金合欢素(11)、异泽兰黄素(12)、槲皮素(13)、香叶木素(14)、木犀草素(15)、芹菜素(16)、芹菜素-7-O-β-D-葡萄糖苷(17)、槲皮素-3-O-β-D-葡萄糖苷(18)、木犀草素-7-O-β-D-葡萄糖苷(19)、芹菜素-7-O-β-D-新橙皮糖苷(20)、金合欢素-7-O-β-D-葡萄糖苷(21)。结果:其中,化合物1~12、18和20为首次从滁菊中分离得到。化合物10、13~16表现出显著的自由基清除能力,活性强于Vit C。
Objective: To study the chemical composition of chrysanthemum leaves. Methods: The ethanol extract of Chrysanthemum morifolium was isolated and purified by silica gel, Sephadex LH-20 and preparative high performance liquid chromatography (HPLC). The structures of compounds were identified by physicochemical properties and spectral data. The DPPH · scavenging ability of the compounds Oxidation ability. RESULTS: Twenty-one compounds were isolated and identified from the ethanol extract of Chrysanthemum morifoliume. They were: Octacosanol (1), β-sitosterol (2), lupuirenol (3) 4, daucosterol (5), ineupatorolide B (6), syringin (7), chlorogenic acid (8), petasiphenol (9), physcion (10) (18), quercetin (13), geraniol (14), luteolin (15), apigenin (16), apigenin-7-O-β-D-glucoside ), Quercetin -3-O-β-D-glucoside (18), luteolin-7-O-β-D-glucoside (19) Hesperidin (20), acacetin-7-O-β-D-glucoside (21). Results: Among them, compounds 1 ~ 12, 18 and 20 were isolated from chrysanthemum for the first time. Compounds 10,13 ~ 16 showed significant free radical scavenging activity, stronger activity than Vit C.