【摘 要】
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Dear Editor,rnTo establish a more specific diagnostic assay than the current methods for rabies virus (RABV) infection in canines and wildlife species,we develo
【机 构】
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Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Laboratory of Epidemiology, Ve
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Dear Editor,rnTo establish a more specific diagnostic assay than the current methods for rabies virus (RABV) infection in canines and wildlife species,we developed a SYBR-Green Ⅰ quantitative real-time reverse transcription-PCR (RTPCR).Three primers were designed and synthesized:one of which,located within the leader sequences and targeted for the whole viral genome amplification to obtain its cDNA,is designated as reverse transcription primer;the other two (as a pair),located within the conserved region of the nucleoprotein gene and aimed at amplifying the nucleoprotein gene,are designated as amplification primers.This real-time RT-PCR can be used for rapid qualitative analysis on dog brain samples and quantitative analysis in fundamental research.
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