论文部分内容阅读
花粉不育是栽培稻(Oryza sativa L.)籼粳亚种间杂种F1普遍存在的现象,是杂种优势利用的主要障碍之一.至少有6个基因座位控制籼粳亚种间F1的花粉不育,各座位存在的花粉育性中性基因可望克服各自的不育性,所以挖掘和利用不同座位的花粉育性中性基因具有重要意义.本文在栽培稻F1花粉不育基因Sb座位已精细定位和广东高州普通野生稻(O.rufipogen Griff.)(以下简称高州野生稻)具有丰富遗传多样性的基础上,分别以粳稻台中65(编号为E1,基因型SbjSbj)及其Sb座位的近等基因系E2(基因型SbiSbi)为母本,12份不同编号的高州野生稻分别为父本,组配成对测交组合并检测各组合F1的花粉育性,初步鉴定可能具有花粉育性中性基因的材料,并发展相应的F2群体,利用4对与Sb座位紧密连锁的分子标记,分析上述成对测交组合F2群体分子标记的分离情况,并与花粉育性的分离进行统计检验.结果表明,编号为GZW099的高州野生稻与E1及E2组配的成对测交组合的F1花粉育性分别为(89.22±1.07)%和(85.65±1.05)%,表现正常可育且经t检验差异不显著;4对分子标记在相应的F2群体中的3种基因型分离比例符合孟德尔分离比例(1:2:1),且3种基因型对应的平均花粉育性差异不显著,说明该编号的野生稻在Sb座位携带的等位基因与台中65及E2的等位基因均不存在显著互作,因此鉴定GZW099在Sb座位携带花粉育性中性基因,命名为SbnSbn,为进一步研究和克服籼粳杂种不育性提供了理论基础和遗传资源.
Pollen sterility is one of the main obstacles to heterosis utilization in hybrid rice F1 between Oryza sativa L. At least 6 loci control pollen F1 between indica and japonica subspecies Therefore, it is of great importance to discover and utilize the pollen fertility neutral genes of different loci.In this paper, the F1 locus of F1 pollen sterility in cultivated rice Based on the rich genetic diversity of O. rufipogen Griff. And high locus O. rufipogen Griff. In Guangdong Province, the genetic diversity of japonica rice Taichung 65 (E1, genotype SbjSbj) and their Sb loci Near-isogenic lines E2 (genotype SbiSbi) were used as female parents. Twelve different populations of Gaozhou wild rice were used as paternal parents, respectively. The pollen fertility of F1 in combination was determined and tested. Sex neutral genes and developed corresponding F2 population. Four pairs of molecular markers tightly linked to Sb locus were used to analyze the segregation of the molecular markers of the F2 populations in the pair-wise cross combinations and to be statistically separated from the pollen fertility Test The results showed that the F1 pollen fertility of F1 hybrids of Gaozhou wild rice with E1 and E2 was numbered as (GZW099) and the pollen fertility of F1 was (89.22 ± 1.07)% and (85.65 ± 1.05)%, respectively. There was no significant difference between the three genotypes in four pairs of F2 markers and the Mendelian segregation ratio (1: 2: 1), and the average pollen fertility of the three genotypes was not significantly different Therefore, it was identified that GZW099 carries a pollen-fertility-neutral gene at Sb locus named SbnSbn, which was further studied in this study And to overcome the indica / japonica hybrid sterility provided the theoretical basis and genetic resources.