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用小球藻(Chlorella sp.)为食培养萼花臂尾轮虫(Brachionus calyciflorus),食物浓度约1×10~6cells/mL,温度25℃,光照强度约40001x,昼长比LD=18:6。在1.0mL、0.5mL和0.25mL培养液中同时进行单个体和群体培养。结果发现:1)单个体培养时每个母体平均产卵量分别为12.0,13.8和7.8个;后代个体中混交雌体的百分比分别是46.38％,53.49％和55.83％。2)群体培养时,每个母体平均产卵量分别为8.7、3.1和2.65个,随密度增加而减少;后代个体中混交雌体百分比分别为41.70％,53.59％和54.26％,群体培养的密度为4.0ind./mL与2.0ind./mL,2.0ind./mL与1.0ind./mL混交雌体的百分比差异不明显。母体密度高低与后代混交雌体百分比间具有明显相关性。在上述培养条件下,轮虫为1.0ind./mL时,进行单个体培养,分别用急剧冷休克(SS)、逐渐冷休克(GS)和恒温对照(C)进行处理,结果表明:个体平均产卵量分别为10.8、9.2和12.6个。后代混交雌体百分比分别为32.85、20.7和16.87,SS和C处间后其混交雌体比例具显著差异,而SS和GS,GS和C处理间没有显著差异。
Brachionus calyciflorus was fed on Chlorella sp., The food concentration was about 1 × 10 ~ 6cells / mL, the temperature was 25 ℃, the light intensity was about 40001x, the day length ratio was LD = 18: 6. Single and group cultures were performed simultaneously in 1.0 mL, 0.5 mL, and 0.25 mL of broth. The results showed that: 1) The average number of fecundity per maternal body was 12.0, 13.8 and 7.8, respectively. The percentages of females in offspring were 46.38%, 53.49% and 55.83%, respectively. 2) The mean fecundity of each maternal population was 8.7, 3.1 and 2.65, respectively, and decreased with the increase of density. The percentage of females in the offspring was 41.70%, 53.59% and 54.26% respectively. The population density There was no significant difference in the percentage of females between 4.0 ind./mL and 2.0 ind./mL, 2.0 ind./mL and 1.0 ind./mL. There was a significant correlation between maternal density and percentage of offspring mixed females. Under the above culture conditions, when rotifer was 1.0 ind./mL, single-body culture was carried out and treated with rapid cold shock (SS), gradual cold shock (GS) and isothermal control (C) respectively. The results showed that: The fecundity was 10.8, 9.2 and 12.6 respectively. The percentages of intercourse females in offspring were 32.85, 20.7 and 16.87, respectively. There were significant differences in the proportions of female and mixed females after SS and C, but no significant differences between SS and GS, GS and C treatments.