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用２．５％戊二醛作偶联剂，分别合成牛血清白蛋白──氨苄西林钠抗原和孵白蛋白──氨苄西林钠抗原．用牛血清白蛋白──氨苄西林钠作免疫原，经免背部多点注射，连续强化免疫五次（每次间隔１月）后，制备免抗氨苄西林钠血清。用饱和硫酸铵法纯化抗血清，获抗氨苄西林钠ＩｇＧ，经酶联免疫测定最佳效价为１：１０００．用卵白蛋白——氨苄西林用作包板抗原，最适浓度为８μｇ／Ｌ，４℃过夜，采用４１℃孵板反应温度，建立了快速测定乳中氨苄西林钠含量的酶联免疫吸附法。氨苄西林钠测定范围５～１６０μｇ／Ｌ，最小检测限为２．５μｇ／Ｌ，回收率为１００％±５％．抗血清亲和系数为１．１４×１０~７，同氨苄西林钠和青毒素Ｇ交叉反应率分别为１００％和９８％。
Using 2.5% glutaraldehyde as coupling agent, bovine serum albumin ─ ─ ampicillin sodium antigen and albumin ─ ─ ampicillin sodium antigen were synthesized. With bovine serum albumin ─ ─ ampicillin sodium as an immunogen, no more back to the injection, continuous immunization five times (each interval of 1 month), the preparation of anti-ampicillin serum anti-free. The antiserum was purified by saturated ammonium sulfate method and got ampicillin sodium IgG. The optimal titer was 1: 1000 by enzyme - linked immunosorbent assay. Using ovalbumin-ampicillin as antigen, the optimal concentration was 8μg / L and overnight at 4 ℃. The enzyme-linked immunosorbent assay (ELISA) was established for rapid determination of ampicillin sodium in milk using 41 ℃ incubation temperature. Ampicillin sodium range of 5 ~ 160μg / L, the minimum detection limit of 2.5μg / L, the recovery rate of 100% ± 5%. The antiserum affinity coefficient was 1.14 × 10 ~ 7, with 100% and 98% cross-reactivity with ampicillin and penicillin G respectively.