本文利用离子色谱—荧光检测法研究同时分离分析多种喹诺酮类广谱抗菌药的方法。氟哌酸、盐酸环丙沙星、依诺沙星在流速为 1ml/min,淋洗液为 0 .0 15 mol/l的 H2 SO43 5 % CH3OH的离子色谱仪能很好的分离。用激发波长为 347nm,发射波长为 42 0 nm的荧光检测器进行检测。三者的检测限分别是 0 .0 5 ppm,0 .10 5 ppm和 0 .0 8ppm。具有良好的重现性和线性关系。对药物样品与混合样品进行测定 ,结果令人满意。 In this paper, ion chromatography - fluorescence detection method for the simultaneous separation and analysis of a wide range of quinolone broad-spectrum antibacterials. Norfloxacin, norfloxacin, was well separated by ion chromatography with norfloxacin at a flow rate of 1 ml / min and with an eluent of 0.015 mol / l of H2 SO43 5% CH3OH. The fluorescence detector with an excitation wavelength of 347 nm and an emission wavelength of 42 0 nm was used for detection. The three detection limits were 0 .0 5 ppm, 0. 10 5 ppm and 0. 08ppm. Has good reproducibility and linearity. The drug samples and mixed samples were measured, the results were satisfactory.