Structural Analysis of an Oligosaccharide and Glycopeptide Mixture from Panax Ginseng Root with Inhi

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A mixture of oligosaccharide and glycopeptide was isolated from the aqueous extract of Panax ginseng roots. The mixture inhibits protein tyrosine phosphatase(SHP-1) function, implying it enhances immune activity. The peak molecular mass of the oligosaccharide portion is 1800 calculated via GPC software after separation by HPLC. And the structure of the oligosaccharide portion is the backbone of (1→3)-and (1→4)-linked arabinopyranoside, and (1→4)-and (1→6)-linked glucopyranoside, with non-reducing terminals of arabinopyranoside and glucopyranoside. The peak molecular mass of glycopeptide portion is 1900 calculated via GPC software after separation by HPLC. The structure of glycopeptide portion is the backbone of (1→3)-and (1→4)-linked arabinopyranoside, and (1→3,6)-linked glucopyranoside, with non-reducing terminals of galactopyranose and glucopyranoside. The peptide composition is Glu, Asp, Hyp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Ile, Leu and Lys. The oligosaccharide-peptide linkage is formed by Ara and Hyp. The mixture of oligosaccharide and glycopeptide was isolated from the aqueous extract of Panax ginseng roots. The mixture inhibits protein tyrosine phosphatase (SHP-1) function, implying it enhances immune activity. The peak molecular mass of the oligosaccharide portion is 1800 calculated via GPC software after separation by HPLC. And the structure of the oligosaccharide portion is the backbone of (1 → 3) -and (1 → 4) -linked arabinopyranoside, and (1 → 4) -and (1 → 6) -linked glucopyranoside, with non-reducing terminals of arabinopyranoside and glucopyranoside. The peak molecular mass of glycopeptide portion is 1900 calculated via GPC software after separation by HPLC. The structure of glycopeptide portion is the backbone of (1 → 3) -and (1 → 4) -linked arabinopyranoside, and (1 → 3,6) -linked glucopyranoside with non-reducing terminals of galactopyranose and glucopyranoside. The peptide composition is Glu, Asp, Hyp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Leu and Lys. The oligosaccharide-pept ide linkage is formed by Ara and Hyp.
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